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KMID : 0881720180330030185
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Journal of Food Hygiene and Safety
2018 Volume.33 No. 3 p.185 ~ p.192
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Development of an Indirect Enzyme-Linked Immunosorbent assay for Rapid Detection of Adulteration of Food Allergen Mackerel in Processed Marine Foods
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Lee Jeong-Eun
Kim Ah-Yoon
Kim Sol-A
Kim Hyo-In
Park Ji-Hye
Shim Won-Bo
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Abstract
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The purpose of this study was to develop an indirect enzyme-linked immunosorbent assay (indirect ELISA) based on a monoclonal antibody (MAb) that is specific to mackerel thermal stable-soluble protein (TSSP), that can be used for the rapid detection of mackerel in processed marine foods. Among the four MAbs (3A5-1, 2, 9, and 12) developed in previous studies, the 3A5-2 MAb that showed high specificity and sensitivity were selected and used to develop the indirect ELISA method. The detection range of the indirect ELISA was 0.02%-0.001% and the detection limit of 0.001% was shown. No cross-reaction to other marine products and food ingredients was observed by the indirect ELISA. Processed marine foods containing mackerel with ≥ 0.3 O.D. value at 405 nm were estimated as positive samples by the indirect ELISA. Therefore, the indirect ELISA can be used as a rapid and sensitive method to identify mackerel authenticity and adulteration in processed marine foods.
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KEYWORD
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Mackerel , Indirect ELISA , Processed marine food , Authenticity , Adulteration
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